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Key Documents

PLA0016

Sigma-Aldrich

Rabbit anti-MDC1 Antibody, Affinity Purified

Powered by Bethyl Laboratories, Inc.

Synonym(s):

Em:AB023051.5, KIAA0170, NFBD1, homologue to Drosophila photoreceptor protein calphotin, mediator of DNA-damage checkpoint 1, nuclear factor with BRCT domains 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

grade

Powered by Bethyl Laboratories, Inc.

species reactivity

human

technique(s)

immunoprecipitation (IP): 2-5 μg/mg
western blot: 1:2000-1:10000

accession no.

NP_055456.1

UniProt accession no.

Q14676

shipped in

wet ice

storage temp.

2-8°C

Gene Information

rabbit ... MDC1(9656)

Immunogen

The epitope recognized by PLA0016 maps to a region between residues 475 and 525 of human Mediator of DNA Damage Checkpoint 1 using the numbering given in entry NP_055456.1 (GeneID 9656).

Physical form

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% sodium azide

Other Notes

MDC1 is required for the S- and G2/M-checkpoint-mediated cell cycle arrest in response to DNA damage. MDC1 interacts with H2AX near sites of DNA double-strand breaks and recruits ATM kinase to foci of DNA damage. MDC1 also appears to regulate the function of other proteins involved in the DNA damage checkpoint such as BRCA1 and Chk2.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Joshua T Burgess et al.
Nucleic acids research, 49(6), 3294-3307 (2021-03-05)
DNA repair pathways are essential to maintain the integrity of the genome and prevent cell death and tumourigenesis. Here, we show that the Barrier-to-Autointegration Factor (Banf1) protein has a role in the repair of DNA double-strand breaks. Banf1 is characterized
Yuki Yamamoto et al.
Cancer research, 74(14), 3707-3715 (2014-05-17)
Failure to expeditiously repair DNA at sites of double-strand breaks (DSB) ultimately is an important etiologic factor in cancer development. NBS1 plays an important role in the cellular response to DSB damage. A rare polymorphic variant of NBS1 that resulted
Bárbara Alcaraz Silva et al.
The Journal of biological chemistry, 289(33), 22771-22784 (2014-07-02)
Chromosome ends contain nucleoprotein structures known as telomeres. Damage to chromosome ends during interphase elicits a DNA damage response (DDR) resulting in cell cycle arrest. However, little is known regarding the signaling from damaged chromosome ends (designated here as "TIPs")
Justin W Leung et al.
PLoS genetics, 10(3), e1004178-e1004178 (2014-03-08)
Histone ubiquitinations are critical for the activation of the DNA damage response (DDR). In particular, RNF168 and RING1B/BMI1 function in the DDR by ubiquitinating H2A/H2AX on Lys-13/15 and Lys-118/119, respectively. However, it remains to be defined how the ubiquitin pathway

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