P7123

Protein Kinase Cν (PKCν) Active human

recombinant, expressed in E. coli, ≥75% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): PKD3, Protein Kinase C nu
• MDL number: MFCD07371119
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• Assay: ≥75% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• mol wt: protein apparent mol wt ~142 kDa
• UniProt accession no.: O94806
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PRKD3(23683)

Description

Application

Kinase activity is measured as the molar amount of phosphate incorporated into the CREBtide substrate peptide (KRREILSRRPSYR) per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

Biochem/physiol Actions

PKCν, also known as PKD3, is a member of the protein kinase C (PKC) family of serine/threonine kinases that play critical roles in the regulation of cellular differentiation and proliferation in many cell types. PKCν is an important physiologic target of the B-cell receptor (BCR) and exhibits activation upon BCR engagement.

Physical form

Solution of 5 μg in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol

Safety Information

• Storage Class Code: 10 - Combustible liquids