P1374

Anti-Pro-BDNF antibody produced in rabbit

affinity isolated antibody, lyophilized powder

• Synonym(s): Anti-pro-Brain-derived Neurotrophic Factor
• MDL number: MFCD08705507
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: mouse, human, rat
• technique(s): western blot: 1:200 using rat glioma C6 and human neuroblastoma SH-SY5Y cell lines; western blot: 1:400 using recombinant mouse proBDNF
• UniProt accession no.: P23560
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... BDNF(627) ; mouse ... Bdnf(12064) ; rat ... Bdnf(24225)

Description

General description

Does not cross react with pro-NGF, pro-NT-3, or mature BDNF.

The brain derived neurotrophic factor (BDNF) gene is mapped to human chromosome 11p14.1. The gene encodes a precursor protein, proBDNF. Mature BDNF (mBDNF) is synthesized by post-translational cleavage of proBDNF. Does not cross react with pro-NGF, pro-NT-3, or mature BDNF.

Immunogen

peptide corresponding to amino acid residues 72-88 of human BDNF (precursor). This sequence has 16/17 residues identical in mouse and rat.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem/physiol Actions

Brain derived neurotrophic factor (BDNF) belongs to a family of secreted proteins called neurotrophins. Neurotrophins are synthesized as preproneurotrophin precursors and further processed to proneurotrophins and subsequently to neurotrophins. BDNF is expressed in the developing and the mature brain. The influence of BDNF is mediated by its binding to TrkB receptor and sometimes the receptor p75^NTR. The downstream pathways promoted by BDNF include PI3K, MAPK and JAK/STAT. The most important role of BDNF is the regulation of synaptic transmission and plasticity, protein synthesis and phosphorylation of local proteins.

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, with 1% bovine serum albumin and ≤0.1% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable