P0270

Calmodulin from bovine heart

lyophilized powder, 2,500-10,000 units/mg protein

• Synonym(s): Phosphodiesterase 3′:5′-cyclic nucleotide activator from bovine heart
• CAS Number: 77107-46-1
• MDL number: MFCD00131869
• UNSPSC Code: 12352202
• NACRES: NA.61

Properties

• biological source: bovine heart
• Quality Level: 200
• form: lyophilized powder
• specific activity: 2,500-10,000 units/mg protein
• composition: Protein, ≥85% Lowry
• technique(s): calcium flux assay: suitable
• UniProt accession no.: P62157
• storage temp.: −20°C
• Gene Information: cow ... CALM3(520277)

Description

Application

This is the protein activator required for the Ca²⁺ dependent activation of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520.

Biochem/physiol Actions

Ca²⁺ binding protein that is required for activation of cyclic nucleotide-dependent phosphodiesterase. It is also a cofactor/activator of nitric oxide synthase, calcineurin, and many kinases including ATPase, myosin light chain kinase, and CAM kinase I, II, and III. It mediates ryanodine receptor activation by cyclic ADP ribose and is involved in intracellular Ca²⁺ homeostasis.

Unit Definition

One unit will stimulate 0.008 activated unit of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520, in a 3 mL reaction volume at pH 7.5 and 30 °C, to 50% of the maximum activity of the enzyme when saturated with activator, in the presence of 0.01 mM Ca²⁺.

Physical form

Lyophilized powder containing buffer salts as imidazole and magnesium sulfate

Preparation Note

Purified by a modification of the procedure of Teo, T.S., et al., J. Biol. Chem., 248, 588 (1973).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves