N9891

Monoclonal Anti-Nebulin antibody produced in mouse

clone NB2, ascites fluid

• Synonym(s): Anti-AMC6, Anti-NEB177D, Anti-NEM2
• MDL number: MFCD00164706
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: NB2, monoclonal
• mol wt: antigen 500 kDa
• contains: 15 mM sodium azide
• species reactivity: vertebrates, chicken
• technique(s): indirect immunofluorescence: 1:400 using frozen sections of animal skeletal muscle; western blot: suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: chicken ... NEB(101748402)

Description

General description

Nebulin is a 700kDa protein which is expressed in skeletal muscles. It possesses 35 amino acid repeats and a C terminal serine-rich and SH3 domains.

Nebulin is also localized to cardiac muscle. Monoclonal Anti-Nebulin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

Localizes nebulin in skeletal muscle of a wide variety of species from cold-blooded vertebrates to man. Does not crossreact with smooth or cardiac muscle, non-muscle tissue and cultured cells. Localizes nebulin within the I band of skeletal muscle sections by indirect immunofluorescence.

Immunogen

chicken breast nebulin.

Application

Monoclonal Anti-Nebulin antibody produced in mouse has been used in:

• immunohistochemistry
• fluorescent immunostaining
• western blotting

Biochem/physiol Actions

Nebulin associates with the Z-line and thin actin filament in a sarcomere. It may function as a regulator of sarcomere thin filament length. The protein also acts as a structural link between sarcomeres. Nebulin interacts with the capping protein CapZ and has a role in controlling the localization of actin filaments.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable