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N7162

Sigma-Aldrich

Anti-Neuroglobin antibody produced in rabbit

~1.2 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Ngb

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About This Item

MDL number:
MFCD09265363
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rat, human, mouse

concentration

~1.2 mg/mL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5-5.0 μg/mL using biotin/ExtrAvidin®-Peroxidase staining of heat-retrieved rat brain sections
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5.0-10.0 μg/mL using biotin/ExtrAvidin®-Peroxidase staining of heat-retrieved human and mouse brain sections

UniProt accession no.

Q9NPG2

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NGB(58157)

General description

Neuroglobin is an oxygen-binding heme protein predominantly expressed in the nervous system in man and other vertebrates. Neuroglobin belongs to the globin superfamily. It displays the typical globin fold, but it shares little amino acid sequence similarity with vertebrate hemoglobins and myoglobins. Neuroglobin is highly conserved among vertebrates and is evolutionary related to the invertebrate nerve globins. In mouse and rat, neuroglobin is preferentially expressed in neuronal cells of the central and peripheral nervous systems, but also in some endocrine tissues. Neuroglobin is highly expressed in neurons of mammalian retina.

Immunogen

synthetic peptide corresponding to amino acids 55-70 of human neuroglobin, conjugated to KLH.

Application

Anti-Neuroglobin antibody produced in rabbit has been used in
  • immunofluorescence double staining
  • immunostaining
  • immunohistochemistry

Biochem/physiol Actions

Neuroglobin may play an essential role in oxygen homeostasis of neuronal cells. Neuroglobin is thought to protect neurons from hypoxic-ischemic injury as suggested by its increased expression under hypoxic conditions in- vitro as well as in focal cerebral ischemia in vivo. Neuronal survival after hypoxia decreased when neuroglobin expression is inhibited by antisense oligonucleotide and enhanced by neuroglobin overexpression. Its distribution correlates with the subcellular localization of mitochondria and with oxygen demands, possibly linking neuroglobin to cellular respiration.

Target description

Neuroglobin is an oxygen-binding heme proteinpredominantly expressed in the nervous system.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Susi Zara et al.
Gerontology, 59(3), 250-260 (2013-02-23)
Alzheimer's disease (AD) is a frequent form of senile dementia. Neuroglobin (Ngb) has a neuroprotective role and decreases Aβ peptide levels. Ngb, promoting Akt phosphorylation, activates cell survival involving cyclic-nucleotide response element-binding protein (CREB). A new molecule (IBU-LA) was synthetized
Divergent Distribution in Vascular and Avascular Mammalian Retinae Links Neuroglobin to Cellular Respiration
Bentmann A, et al.
The Journal of Biological Chemistry, 280(21), 20660-20665 (2005)
Zindy Raida et al.
Experimental & translational stroke medicine, 4(1), 15-15 (2012-08-21)
Neuroglobin is considered to be a novel important pharmacological target in combating stroke and neurodegenerative disorders, although the mechanism by which this protection is accomplished remains an enigma. We hypothesized that if neuroglobin is directly involved in neuroprotection, then permanent
Neuroglobin involvement in visual pathways through the optic nerve
Lechauve C, et al.
Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1834(9), 1772-1778 (2013)
A vertebrate globin expressed in the brain
Burmester T, et al.
Nature, 407(6803), 520-523 (2000)
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