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N5162

Sigma-Aldrich

Anti-Neurabin II antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Neural-tissue Specific F-actin Binding Protein I, Anti-PP1bp134, Anti-Protein Phosphatase 1 Regulatory Subunit 9B, Anti-Spinophilin

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About This Item

MDL number:
MFCD02686629
UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 140 kDa

species reactivity

rat, mouse, canine

technique(s)

immunoprecipitation (IP): 5-10 μg using rat brain extract (S1 fraction)
indirect immunofluorescence: 5-10 using MDCK cells.
microarray: suitable
western blot: 0.5-1 μg/mL using mouse and rat brain extract (S1 fraction)

UniProt accession no.

Q6R891

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PPP1R9B(84687)
mouse ... Ppp1r9b(217124)
rat ... Ppp1r9b(84686)

Immunogen

synthetic peptide corresponding to amino acids 367-390 located at the mid-region of rat neurabin II. The sequence is identical in human and mouse neurabin II and not found in neurabin I.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Linda C Hsieh-Wilson et al.
The Journal of biological chemistry, 278(2), 1186-1194 (2002-11-06)
Spinophilin is a protein phosphatase 1 (PP1)- and actin-binding protein that modulates excitatory synaptic transmission and dendritic spine morphology. We report that spinophilin is phosphorylated in vitro by protein kinase A (PKA). Phosphorylation of spinophilin was stimulated by treatment of
Madepalli K Lakshmana et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 26(5), 2072-2083 (2012-02-02)
We previously reported that RanBP9 binds low-density lipoprotein receptor-related protein (LRP), amyloid precursor protein (APP), and BACE1 and robustly increased Aβ generation in a variety of cell lines and primary neuronal cultures. To confirm the physiological/ pathological significance of this
H Nakanishi et al.
The Journal of cell biology, 139(4), 951-961 (1997-12-31)
We purified from rat brain a novel actin filament (F-actin)-binding protein of approximately 180 kD (p180), which was specifically expressed in neural tissue. We named p180 neurabin (neural tissue-specific F-actin- binding protein). We moreover cloned the cDNA of neurabin from
Pranay Bharadwaj et al.
PloS one, 8(7), e69672-e69672 (2013-07-23)
Male sexual behavior (MSB) is modulated by gonadal steroids, yet this relationship is highly variable across species and between individuals. A significant percentage (~30%) of B6D2F1 hybrid male mice demonstrate MSB after long-term orchidectomy (herein after referred to as "maters")
C L Pang et al.
Oncogene, 33(31), 4039-4049 (2013-10-22)
High-risk human papillomaviruses are causative agents of cervical cancer. Viral protein E7 is required to establish and maintain the pro-oncogenic phenotype in infected cells, but the molecular mechanisms by which E7 promotes carcinogenesis are only partially understood. Our transcriptome analyses

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