N4662

Nek6, Active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): Gene A-Related Kinase 6, NIMA-Related Kinase 6, Never in Mitosis
• MDL number: MFCD07370972
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli
• Assay: ≥80% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• enzyme activity: ≥10 units/mg protein
• mol wt: protein apparent mol wt ~58 kDa
• UniProt accession no.: Q9HC98
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... NEK6(10783)

Description

Application

Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

Biochem/physiol Actions

Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. It is phosphorylated and activated during M phase of the cell cycle. Inhibition of Nek6 arrests cells in M phase and triggers apoptosis, suggesting that Nek6 is required for metaphase-anaphase transition.

Unit Definition

One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP

Physical form

Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable