N2889

Neutral Red Solution (0.33%)

3.3 g/L in DPBS, sterile-filtered, BioReagent, suitable for cell culture

• Synonym(s): Neutral Red solution, 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Toluylene red
• Empirical Formula (Hill Notation): C₁₅H₁₇ClN₄
• CAS Number: 553-24-2
• Molecular Weight: 288.78
• MDL number: MFCD00012651
• UNSPSC Code: 12352207
• PubChem Substance ID: 24897603
• NACRES: NA.75

Properties

• sterility: sterile-filtered
• product line: BioReagent
• form: solution
• concentration: 3.3 g/L in DPBS
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• storage temp.: 2-8°C
• SMILES string: [Cl-].Cc1cc2N=C3C=C\C(C=C3Nc2cc1N)=[N+](/C)C
• InChI: 1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H
• InChI key: PGSADBUBUOPOJS-UHFFFAOYSA-N

Description

General description

Neutral Red is a cationic dye that is used for nuclear counterstaining in biological research. It also serves as a pH indicator in analytical laboratories. On decomposition, neutral red dye releases carbon monoxide (CO), carbon dioxide (CO₂), nitrogen oxides, and hydrogen chlorides. Therefore, this dye is considered toxic and is recommended to remove before discharge into receiving streams.

Application

Neutral Red Solution (0.33%) has been to stain viable cells and measure cell viability. It has also been used as a component of culture medium for enzymatic digestion of ovarian medulla tissue to extract follicles.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable