MAK435

Acetylcholine Assay Kit

Sufficient for 100 colorimetric or fluorometric tests

• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 100
• input: cell culture(s); plasma; saliva; urine; milk; serum; tissue
• application(s): pharmaceutical
• detection method: colorimetric; fluorometric
• relevant disease(s): cardiovascular diseases, Alzheimer′s disease; neurodegenerative disorders; neurological disorders
• storage temp.: −20°C

Description

General description

Acetylcholine is a multifunctional neurochemical with diverse roles in the brain and various organ systems. It acts as a neurotransmitter, facilitating communication between neurons, myocytes, and cells in glandular tissues. The name "acetylcholine" originates from its chemical composition, which is an ester of acetic acid and choline. Tissues that utilize or respond to acetylcholine are termed cholinergic. While it functions as a neurotransmitter throughout the body, acetylcholine is particularly associated with the neuromuscular junction, where it triggers muscle activation by connecting motor neurons in the ventral spinal cord with muscles. Additionally, acetylcholine serves as a neurotransmitter in the autonomic nervous system, enabling communication between preganglionic and postganglionic neurons. It is the final product released by parasympathetic postganglionic neurons and plays vital roles in skeletal muscle movement, regulation of smooth and cardiac muscle, as well as in learning, memory, and mood.

Application

The Acetylcholine Assay Kit may be used for:

• Neurological Research
• Drug Discovery
• Cell culture analysis

Features and Benefits

Convenient and Sensitive Measurement:

• Utilizes only 20 μL of the sample, providing an accurate determination of acetylcholine.
• Offers a wide detection range of 10-200 μM (colorimetric) and 0.4-10 μM (fluorometric), ensuring sensitivity across varying concentrations.

Simplified Process:

• Enjoy a hassle-free procedure requiring the addition of a single working reagent.
• Complete the assay with just a 30-minute reaction time, saving valuable time and effort.

Suitability

The kit is suitable for the quantitative determination of acetylcholine and evaluation of drug effects on acetylcholine metabolism in serum, plasma, urine, saliva, milk, tissue, and cell culture etc.

Principle

The Acetylcholine Assay Kit provides a simple, direct and high-throughput assay for measuring acetylcholine in biological samples. In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase tobetaine and H₂O₂. The resulting H₂O₂ reacts with a specific dye to form a pink colored product. The color intensity at 570 nm or fluorescence intensity at λ_Ex = 530 nm/λ_Em = 585 nm is directly proportional to the acetylcholine concentration in the sample.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Safety Information

• Hazard Statements: H412
• Precautionary Statements: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): 188.6 °F
• Flash Point(C): 87 °C