MAK142
Fluorometric Intracellular Ros Kit
sufficient for 200 fluorometric tests (Deep red)
Synonym(s):
Intracellular ROS Fluorescence Assay
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About This Item
usage
sufficient for 200 fluorometric tests (Deep red)
application(s)
cosmetics
food and beverages
detection method
fluorometric
relevant disease(s)
neurological disorders; cancer
storage temp.
−20°C
General description
Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.
Application
- Diethyl Blechnic Exhibits Anti-Inflammatory and Antioxidative Activity via the TLR4/MyD88 Signaling Pathway in LPS-Stimulated RAW264.7 Cells.: This article uses fluorometric methods to explore the anti-inflammatory and antioxidative properties of diethyl blechnic, illustrating the role of ROS assays in elucidating the molecular mechanisms underlying the therapeutic effects of natural compounds (Yang S et al., 2019).
Suitability
This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader, flow cytometer, or a fluorescent microscope with TRITC filter.
Principle
The Fluorometric Intracellular ROS Kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells after 1 hour incubation. ROS react with a fluorogenic sensor localized to the cytoplasm, resulting in a fluorometric product proportional to the amount of ROS present. The assay can be performed in either a 96 or 384 multiwell plate format and with detection at λex = 650/λem = 675 nm.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
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