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HPA026828

Sigma-Aldrich

Anti-RPN1 antibody produced in rabbit

enhanced validation

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-FM-3, Anti-GPC-R, Anti-GPR66, Anti-NMU1R, Anti-OST1, Anti-ribophorin I

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About This Item

UNSPSC Code:
12352203
Human Protein Atlas Number:
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:50-1:200

immunogen sequence

KVHYENNSPFLTITSMTRVIEVSHWGNIAVEENVDLKHTGAVLKGPFSRYDYQRQPDSGISSIRSFKTILPAAAQDVYYRDEIGNVSTSHLLILDDSVEMEIRPRFPLFGGWKTHYIVGYNLPSYEYLYNLGDQYALKMRFV

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RPN1(6184)

General description

Ribophorin I (RPN1) is a type I transmembrane glycoprotein of 583 amino acids encoded by the gene mapped to human chromosome 3q21. It is one of the subunits of oligosaccharyltransferase (OST) complex expressed predominantly in endoplasmic reticulum membrane. The encoded protein is characterized with leucine-rich-repeat-like (LRR-like) domain involved in binding several ligands to the proteasome.

Immunogen

ribophorin I recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information.

Biochem/physiol Actions

Ribophorin I (RPN1) promotes N- glycosylation of potential substrates, which are near to the catalytic subunit of the oligosaccharyltransferase (OST) complex. RPN1 functions as a part of regulatory subunit of proteasome and helps in binding proteins with ubiquitin-like (UBL) domains to the proteasome. The encoded protein might associate with signal recognition complexes and facilitate the transfer of secretory and membrane proteins across the microsomal membrane. Ribophorin I directly interacts with μ-opioid receptor (MOR) and modulates its cell surface expression. This protein associates with misfolded ribonuclease A, but not with the natural form, proposing that it might act as a chaperone that identifies misfolded proteins inside cells.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST86863

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide.

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Activation of a novel gene in 3q21 and identification of intergenic fusion transcripts with ecotropic viral insertion site I in leukemia.
Pekarsky Y
Cancer Research, 57(18), 3914-3919 (1997)
Ribophorin I regulates substrate delivery to the oligosaccharyltransferase core.
Proceedings of the National Academy of Sciences of the USA, 105(28), 9534-9539 (2008)
mu-Opioid receptor cell surface expression is regulated by its direct interaction with Ribophorin I.
Ge X
Molecular Pharmacology, 75(6), 1307-1316 (2009)
Proteasome subunit Rpn1 binds ubiquitin-like protein domains.
Nature Cell Biology, 4(9), 725-730 (2002)
Identification of a breakpoint cluster region 3' of the ribophorin I gene at 3q21 associated with the transcriptional activation of the EVI1 gene in acute myelogenous leukemias with inv(3)(q21q26).
Suzukawa K
Blood, 84(8), 2681-2688 (1994)

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