H4641
Hanks′ Balanced Salt Solution 10x
Without calcium chloride, magnesium sulfate and sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Hanks’ Balanced Salt solution, HBSS
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Quality Level
sterility
sterile-filtered
form
liquid
concentration
10 ×
technique(s)
cell culture | mammalian: suitable
components
phenol red: yes
shipped in
ambient
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General description
Balanced salt solution (BSS) is crucial in cell culture as it serves as an irrigating, transporting, and diluting agent to maintain the intra- and extracellular osmotic balance and also provides energy for cell metabolism. It provides water and bulk inorganic ions to the cells to maintain the normal cell metabolism. BSS plays a role in the buffering system to maintain the medium within the physiological pH range (7.2-7.6).
Application
Hanks′ Balanced Salt Solution 10x has been used:
- as a diluting agent during the Chinese hamster ovary cell line detachment from growth medium
- to wash the biopsy specimens of lamina propria lymphocytes (LPLs)
- as a wash solution to wash the fish cell line during removal from growth medium
Reconstitution
Supplement with 0.35 g/L sodium bicarbonate at 1X.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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