Skip to Content
MilliporeSigma
All Photos(4)

Documents

H1650

Sigma-Aldrich

Monoclonal Anti-HLA Class I Antigen antibody produced in mouse

clone W6/32, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-HLA Class I Antigen

Sign Into View Organizational & Contract Pricing
All Photos(4)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

W6/32, monoclonal

form

buffered aqueous solution

species reactivity

monkey, human

technique(s)

flow cytometry: 5 μL using 1 × 106 cells
immunoprecipitation (IP): suitable
indirect immunofluorescence: 1:10 using acetone-fixed human tonsil frozen sections.

isotype

IgG2a

UniProt accession no.

P04439

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... HLA-A(3105) , HLA-B(3106) , HLA-C(3107)

Looking for similar products? Visit Product Comparison Guide

General description

Class I proteins are highly polymorphic, in which HLA-B seems to be the most polymorphic gene in the human genome. HLA-A, -B, -C are classical class I proteins and are heterodimers. HLA-A, -B, -C consists of a polymorphic transmembrane α heavy chain and an invariant water-soluble light chain. HLA-B is located on human chromosome 6p21.

The previously assigned protein identifier P01891 has been merged into P04439. Full details can be found on the UniProt database.

Specificity

Recognizes the human HLA Class I antigen expressed on most human nucleated cells. The antibody reacts with a monomorphic epitope on the 44-45 kDa α-chain polypeptide of human class I HLA molecules (HLA-A,B,C). The antibody may be used in immunoprecipitation, complement mediated cytotoxicity, flow cytometry and immunohistochemical staining of acetone-fixed, frozen sections and cell smears. It cross-reacts with cells from African and Asian apes and old world monkeys. No reactivity is seen with cells from most new world monkeys or from non-primates. The epitope recognized by this antibody is sensitive to routine formalin fixation and paraffin embedding.
4th Workshop: code no. P1
5th Workshop: code nos. BP166, BP288, BP407, B 005

Immunogen

human tonsil leukocyte membrane preparation.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem/physiol Actions

HLA class I molecules like HLA-A, -B and −C help in the presentation of endogenous antigen to CD8+ (cluster of differentiation 8) T cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Apurva R Patel et al.
Pharmaceutical research, 31(10), 2796-2809 (2014-05-29)
Evaluation of tumor targeting pegylated EphA2 peptide coated nanoparticles (ENDDs) of a novel anticancer agent DIM-C-pPhC6H5 (DIM-P) and Docetaxel (DOC) and investigate its antitumor activity and potential for treatment of lung cancer. Nanoparticles were prepared with DIM-P and DOC (NDDs)
Major histocompatibility complex class II
The Systematic Identification of Organic Compounds, 3-19 (2011)
The human gene map for performance and health-related fitness phenotypes: the 2005 update
Rankinen T, et al.
Med Sci Sports Exerc., 38(11), 1863-1888 (2006)
Kian Kani et al.
Molecular cancer therapeutics, 11(5), 1071-1081 (2012-03-14)
Clinical oncology is hampered by lack of tools to accurately assess a patient's response to pathway-targeted therapies. Serum and tumor cell surface proteins whose abundance, or change in abundance in response to therapy, differentiates patients responding to a therapy from
Dong Hao Jin et al.
Oncotarget, 8(60), 101509-101519 (2017-12-20)
A target molecule responsible for cell cycle arrest by metformin was discovered using a gene chip array in lung cancer cells and the effect of metformin on E2F8 was assessed. The siRNA-mediated knockdown of E2F8 significantly suppressed G1-S progression while
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service