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Key Documents

GE17-0969-01

IgG Sepharose™ 6 Fast Flow

Cytiva 17-0969-01, pack of 10 mL

Synonym(s):

IgG Column

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

human IgG

packaging

pack of 10 mL

manufacturer/tradename

Cytiva 17-0969-01

storage condition

(20% Ethanol, 50 mM K2PO4, 1mM MgCl2 pH 7.2 in 20% Ethanol)

matrix

highly cross-linked 6% agarose

average diameter

90 μm

cleaning in place

3-12

working range

3-10

capacity

~3 mg binding capacity(Protein A)

storage temp.

2-8°C

General description

lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose™ 6 Fast Flow matrix, with human IgG covalently coupled to it. Medium allows high flow rates to be used for rapid and convenient single step purification of protein.

lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose™ 6 Fast Flow matrix, with human IgG covalently coupled to it. The improved mechanical characteristics of this Fast Flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.

The mechanical characteristics of this fast flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.

Features and Benefits

  • IgG coupled to the highly cross-linked 6% agarose matrix Sepharose™ 6 Fast Flow, designed for the rapid purification of protein A and protein A fusion conjugates
  • Binds at least 2 mg protein A/mL medium
  • Suitable for tandem affinity purification (TAP) of protein complexes

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocols

Purification of Protein A-Tagged Proteins

This page shows how to purify protein A-tagged proteins using GE Healthcare products.

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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Contact Technical Service