G9880

Glutamic-Pyruvic Transaminase from porcine heart

ammonium sulfate suspension, ≥75 units/mg protein

• Synonym(s): L-Alanine: 2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT
• CAS Number: 9000-86-6
• Enzyme Commission number: 2.6.1.2 (BRENDA, IUBMB)
• Beilstein: 3562140
• EC Number: 232-561-4
• MDL number: MFCD00131192
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Porcine heart
• Quality Level: 200
• form: ammonium sulfate suspension
• specific activity: ≥75 units/mg protein
• mol wt: 42 kDa
• foreign activity: L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%; glutamic-oxalacetic transaminase ≤0.05%
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Biochem/physiol Actions

Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.

Unit Definition

One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.

Physical form

Suspension in 1.8 M (NH₄)₂SO₄ solution, pH 6.0

Analysis Note

Protein determined by biuret.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves