G5671

Anti-Galectin-8 antibody, Mouse monoclonal

~1.0 mg/mL, clone VA-11.25, purified from hybridoma cell culture

• Synonym(s): ANTIGal-8, ANTILGALS8, ANTILectin, galectoside-binding, soluble 8, ANTIPCTA1, ANTIProstate carcinoma tumor antigen 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: VA-11.25, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~34 kDa
• species reactivity: mouse, rat, human
• concentration: ~1.0 mg/mL
• technique(s): immunocytochemistry: suitable; indirect ELISA: suitable; western blot: 1-2 μg/mL using rat galectin 8 recombinant protein
• isotype: IgM
• UniProt accession no.: O00214
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... LGALS8(3964) ; mouse ... Lgals8(56048) ; rat ... Lgals8(116641)

Description

General description

Galectins specifically bind to β-galactosides. 15 mammalian galectins have been identified so far. Galectin 8 contains two CRDs connected by a short linker peptide.

Monoclonal Anti-Galectin 8 (mouse IgM isotype) is derived from the hybridoma VA-11.25 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant rat galectin 8.Galectins belong to a family of carbohydrate-binding proteins. Within this family of proteins, galectin 8 is unique, as it exists in many forms encoded by the same gene.

Specificity

The antibody has low cross-reactivity with galectin 3 and galectin 9.

Immunogen

recombinant rat galectin 8.

Application

Monoclonal Anti-galectin-8 antibody produced in mouse has been used for:

• immunoblotting
• immunocytochemistry
• enzyme linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Galectins belong to a family of carbohydrate-binding proteins. These proteins share similarities in the carbohydrate recognition domain (CRD) and in their specificity for N-Acetyllactosamine-enriched glycoconjugates. The binding of galectins to saccharides modulates cell proliferation, cell death and cell migration, which are involved in cancer initiation and progression. Galectin 8 is a secreted protein that requires both of its CRDs to be active to modulate cell adhesion. It forms a complex with integrins and triggers integrin-mediated signaling cascades. On the contrary, excessive amounts of this protein negatively regulate cell adhesion by interacting with integrins. These signals assist in identifying regions available for cell adhesion and migration. It may be involved in neoplastic transformation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves