G5422

β-Glycerophosphate disodium salt hydrate

≤1.0 mol % L-α-isomer

• Synonym(s): BGP, Glycerol 2-phosphate disodium salt hydrate
• Linear Formula: (HOCH₂)₂CHOP(O)(ONa)₂ · xH₂O
• CAS Number: 154804-51-0
• Molecular Weight: 216.04 (anhydrous basis)
• EC Number: 212-464-3
• MDL number: MFCD00149082
• UNSPSC Code: 12352201
• PubChem Substance ID: 329799941
• NACRES: NA.25

Properties

• biological source: synthetic
• form: powder
• impurities: ≤0.1% Inorganic phosphorus; ≤1.0 mol % L-α-isomer
• mp: 102-104 °C (lit.)
• solubility: water: 50 mg/mL, clear, colorless
• SMILES string: O.[Na+].[Na+].OCC(CO)OP([O-])([O-])=O
• InChI: 1S/C3H9O6P.2Na.H2O/c4-1-3(2-5)9-10(6,7)8;;;/h3-5H,1-2H2,(H2,6,7,8);;;1H2/q;2*+1;/p-2
• InChI key: ROPZSVKNEIIIDE-UHFFFAOYSA-L

Description

Application

β-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. BGP is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression.

β-glycerophosphate is used in homegenization solutions for muscles extracts following fixation.

Initiates differentiation of bone-marrow-derived stem cells embedded in a natural collagen/chitosan gel designed for tissue engineering.

Quality

Low inorganic phosphate content. Mixtures of α- and β-glycerophosphate have historically been used in microbiological media but high levels of inorganic (free) phosphate has been associated with the irregular growth of bacteria and unwanted precipitates.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves