F8888
Anti-Chicken IgY (IgG) (whole molecule)−FITC antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Rabbit Anti-Chicken IgY (IgG) (whole molecule)−Fluorescein isothiocyanate
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About This Item
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biological source
rabbit
Quality Level
conjugate
FITC conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
storage condition
protect from light
technique(s)
direct immunofluorescence: 1:320 using human peripheral blood lymphocytes
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320 using human tonsil
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Birds, reptiles and amphibians express IgY antibodies that are functionally equivalent to IgG. Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody is specific for IgY of chicken. Rabbit Anti- Chicken IgY (IgG) is conjugated to fluorescein isothiocyanate (FITC). Free FITC is removed by gel filtration.
Chicken IgY is made of two heavy (H) and two light (L) chains. Its molecular weight is about ~180 kDa.
Specificity
Binds chicken IgY (IgG). Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody is specific for chicken IgY (IgG).
Immunogen
Purified chicken IgY (IgG)
chicken IgY (IgG)
Application
Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody has been used in immunofluorescence.
Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody may be used for immunofluorescence using human peripheral blood lymphocytes at a working antibody dilution of 1:320. For immunohistochemistry a working antibody dilution of 1:320 may be used. A dilution of 1:1000 was used for EP receptor staining of murine keratinocyte cell line 308 using primary antibodies generated in chicken. The antibody was also used for immune electron microscopy labeling of porcine nerve samples.
Biochem/physiol Actions
Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus chicken antibodies are useful analytical tools for various research applications .
IgY plays a major role in defense mechanism against systemic infections. It also possesses the ability to initiate anaphylactic reactions.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Characterization of two recombinant HVT-IBD vaccines by VP2 insert detection and cell-mediated immunity after vaccination of specific pathogen-free chickens
Ingrao F, et al.
Avian Pathology : Journal of the W.V.P.A, 46(3), 289-299 (2017)
E J Thompson et al.
Neoplasia (New York, N.Y.), 3(5), 402-410 (2001-11-01)
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G W Warr et al.
Immunology today, 16(8), 392-398 (1995-08-01)
IgY is the functional equivalent of IgG in birds, reptiles and amphibia, but many aspects of its biology are poorly understood. Recent studies have increased awareness of the genetics and functions of this molecule, and have revealed its position as
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