E0760

Endothelial cell growth supplement from bovine pituitary

ECGS, suitable for cell culture, BioReagent

• Synonym(s): ECGS, Endothelial Mitogen
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: bovine pituitary glands
• Quality Level: 200
• product line: BioReagent
• form: powder
• potency: 2-300 μg/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• packaging: pkg of 15 mg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤20 EU/vial (LAL test)
• color: brown to tan
• solubility: water: soluble to hazy, red to red-brown (1 vial reconstituted with 1 ml 0.85% NaCl)
• storage temp.: 2-8°C

Description

General description

Endothelial cell growth supplement (ECGS) is an extract of bovine pituitary glands containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of a published method. It is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.

Application

Endothelial cell growth supplement from bovine pituitary has been used:

• as a component in Roswell park memorial institute (RPMI) 1640 medium for endothelial cell culture
• as a supplement in medium 199 for endothelial cell culture
• as a supplement in Dulbecco′s modified eagle medium (DMEM):Ham′s F-12
• as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) for human umbilical vein endothelial cells (HUVEC) culture

Biochem/physiol Actions

Endothelial cell growth supplement (ECGS) has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.

Safety Information

• Pictograms: GHS08
• Signal Word: Warning
• Hazard Statements: H361fd
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable