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D2158

Sigma-Aldrich

Histodenz™

nonionic density gradient medium

Synonym(s):

5-(N-2,3-Dihydroxypropylacetamido)-2,4,6-triiodo-N,N′-bis(2,3-dihydroxypropyl)isophthalamide

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About This Item

Empirical Formula (Hill Notation):
C19H26I3N3O9
CAS Number:
66108-95-0
Molecular Weight:
821.14
Beilstein:
2406632
EC Number:
266-164-2
MDL number:
MFCD00077732
UNSPSC Code:
12352207
PubChem Substance ID:
24893646
NACRES:
NA.75

form

powder

application(s)

hematology
histology

storage temp.

room temp

SMILES string

CC(=O)N(CC(O)CO)c1c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c1I

InChI

1S/C19H26I3N3O9/c1-8(29)25(4-11(32)7-28)17-15(21)12(18(33)23-2-9(30)5-26)14(20)13(16(17)22)19(34)24-3-10(31)6-27/h9-11,26-28,30-32H,2-7H2,1H3,(H,23,33)(H,24,34)

InChI key

NTHXOOBQLCIOLC-UHFFFAOYSA-N

General description

HistoDenz™, also known as iohexol, is a non-ionic X-ray contrast compound developed specifically as an iodinated density gradient medium.

Application

HistoDenz™ is suitable for separating everything from macromolecules to viable cells by either rate-zonal or isopycnic centrifugation.
  • It has been used in the isolation and culturing of hepatic stellate cells.
  • It has also been employed in the extraction of microbial cells from environmental samples.
  • It has been used in LacZ (β-galactosidase) staining.
  • It has also been used for the enrichment of gonocytes.

Features and Benefits

HistoDenz™ offers several favorable properties as a gradient medium.
  • It has very low toxicity, low osmolality, high density, and low viscosity (compared to sucrose).
  • It is water-soluble, non-ionic, and chemically near-inert.

Legal Information

Histodenz is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Skeletal muscle tissue clearing for lacz and fluorescent reporters, and immunofluorescence staining.
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