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CLLS1214

Sigma-Aldrich

MCF10A CELLS HER2 (-/-) EXON 2

human female maMilliporeary glands (Source Disease: Fibrocystic Disease)

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

product name

MCF10A CELLS HER2 (-/-) EXON 2,

biological source

human female mammary glands (Source Disease: Fibrocystic Disease)

Quality Level

OMIM accession no.

storage temp.

−196°C

Gene Information

General description

MCF10A CELLS HER2 (-/-) EXON 2 are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN modification.

This cell line was derived from ATCC Catalog No. CRL-10317.

Application

Media Renewal changes two to three times per week.

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3-1:8

The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat. No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat. No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat. No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat. No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat. No. H6909, to a final concentration of 0.5 ug/mL.

Cell freezing medium - DMSO 1X, Cat. No. C6164.

Features and Benefits

These MCF10A cells are adherent with a doubling time of approx. 20 hours

Zinc finger nuclease (ZFN) modification on chromosome 17q21.1

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Kit Components Only

Product No.
Description

  • MCF10A CELLS HER2 (-/-) EXON 2 1 vial

Kit Components Also Available Separately

Product No.
Description
SDS

  • MCF10A CONTROL CELLS 1 vial

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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We have applied our revolutionary CompoZr Zinc Finger Nuclease technology to create an unparalleled range of genetically modified mammalian cell lines for use in areas, such as target validation, drug discovery and drug development.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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