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biological source
human cervix
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
EGF, TGF-beta
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human cervix carcinoma
Cell Line Description
The cell line HSG was originally thought to be established from a human submandibular gland from a 54 year old Japanese male. The cell line has since been found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.
Culture Medium
MEM +2mM Glutamine +1% Non Essential Amino Acids (NEAA) + 10% New Born Calf Serum (NBCS).
Subculture Routine
Split confluent cultures 1:4 to 1:10 ie seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2, 37°C
Other Notes
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Certificates of Analysis (COA)
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