G-402

90112715, human kidney, Epithelial-like

• UNSPSC Code: 41106514

Properties

• product name: G-402, 90112715
• biological source: human kidney
• growth mode: Adherent
• karyotype: Modal no. 47
• morphology: Epithelial-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian renal leiomyoblastoma

Cell Line Description

Derived from a tumour of a 9 month old female Caucasian. Highly transformed and grows in soft agar.

Application

Transformation and tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 9,12
D16S539: 11,13
D5S818: 11,12
D7S820: 9,10
THO1: 7,9
TPOX: 10
vWA: 13,17

Culture Medium

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Change medium every 4 days.

Other Notes

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