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product name
CHP 4 (W.W.), 90102537
biological source
human skin
growth mode
Adherent
karyotype
2n = 46
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human skin asymptomatic Galactosemia
Cell Line Description
Derived from skin tissue taken from an 8 year old male with asymptomatic galactosemia, despite lacking red cell galactose-1-phosphate uridyl transferase. Sibling of CHP 3 (M.W.) (Sigma catalogue no. 90102536) with the same enzyme deficiency that was symptomatic. Cultures will retain characteristics for at least 30 passages. Ethnicity: Black.
Application
Enzyme deficiency
Culture Medium
DMEM + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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