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product name
C127I, 90060504
biological source
mouse breast
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse RIII mammary tumour
Cell Line Description
Non-transformed clone from a RIII mouse. Supernatant fluid is negative for RT activity. Useful for visualisation of sarcoma virus-induced foci, in vitro quantitative assay for bovine papilloma virus. Suitable host for transformation with bovine papilloma virus DNA plasmids.
Application
Virus and transformation studies: Sarcoma virus, Bovine papilloma virus
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 to 1:15 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
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