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biological source
human nerve (supraorbital lymph node metastasis)
growth mode
Adherent
karyotype
2n = 46, Hypodiploid to pseudodiploid, abnormalities
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human neuroepithelioma, metastasis to supra-orbital area
Cell Line Description
Reported to have moderate dopamine-β-hydroxylase activity. Formaldehyde-induced fluorescence indicative of intracellular catecholamines.
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:10 to 1:20 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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