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Key Documents

SCP

89101302

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About This Item

UNSPSC Code:
41106514

biological source

ovine brain

growth mode

Adherent

karyotype

Not specified

morphology

Not specified

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Ovine brain choroid plexus

Cell Line Description

Initiated from the choroid plexus of an ovine brain. The cells support propagation of Visna virus, strain 1514 with resulting cytopathic effects. Tests have indicated that the cell line produces both detectable BVDV antigens and infectious BVDV.

Application

Virus studies: Visna virus

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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