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product name
CCRF-SB, 89090405
biological source
human blood
growth mode
Suspension
karyotype
2n = 46
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian acute lymphoblastic leukaemia
Cell Line Description
A B lymphoblastoid line obtained from the peripheral blood of an 11 year old boy with acute lymphoblastic leukaemia. Appear free of virus particles by electron microscopy. Do not synthesise immunoglobulins. Grown in continuous spinner culture for 6 months. HLA profile: A1, A2, B12, B17, Cw2.
Application
HLA profile studies
Culture Medium
RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3x10,000 to 1x100,000 cells/ml; 5% CO2; 37°C. Cultures can tolerate high densities i.e. 1-2x1,000,000 cells/ml.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
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