U-87 MG Cell Line human

from human brain(glioblastoma astrocytoma), 89081402

• Synonym(s): U-87MG Cells, U87 Cells, U87MG Cells
• UNSPSC Code: 41106514

Properties

• biological source: human brain (glioblastoma astrocytoma)
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Epithelial-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human glioblastoma astrocytoma

Cell Line Description

Derived from a malignant glioma from a female patient by explant technique. It is reported to produce a malignant tumour consistent with glioblastoma in nude mice.

Application

Neural studies

U-87 MG cell line has been used to study the effects of the lysosomal destabilizing drug Siramesine on glioblastoma. It has also been used to study calcium-independent disruption of microtubule dynamics by nanosecond pulsed electric fields.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8,11
D16S539: 12
D5S818: 11,12
D7S820: 8,9
THO1: 9.3
TPOX: 8
vWA: 15,17

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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