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product name
LC540, 89031604
biological source
rat testis
growth mode
Adherent
karyotype
2n = 42
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat Fischer Leydig cell testicular tumour
Cell Line Description
A cell line from a spontaneous transplantable Leydig cell tumour in a Fischer strain rat, reported to secrete steroid, however over a 6 hour period the secretion of oestradiol and testosterone by radioimmunoassay could not be confirmed.
Application
Tumourigenicity studies
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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