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Key Documents

BW5147.G.1.4.OUAR.1

88100507, mouse blood, Lymphoblast

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About This Item

UNSPSC Code:
41106514

product name

BW5147.G.1.4.OUAR.1, 88100507

biological source

mouse blood

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse AKR/J T cell lymphoma

Cell Line Description

This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100βM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids.

Application

Resistance and hybridisation studies

Culture Medium

DMEM + 2mM Glutamine + 10% Horse Serum (HS) or 5% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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