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product name
A 172, 88062428
biological source
human nerve
growth mode
Adherent
karyotype
n = 80, over 20 marker chromosomes per cell (hypertriploid)
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human glioblastoma
Cell Line Description
Derived from a glioblastoma removed from a 53 year old male. Non-tumourigenic in anti-thymocyte serum treated NIH Swiss mice.
Application
Tumourigenicity studies
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C (medium renewal every 2-3 days recommended).
Other Notes
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Certificates of Analysis (COA)
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