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product name
RPMI 6666, 87081807
biological source
human blood
growth mode
Suspension
karyotype
2n = 46
morphology
Lymphoblast
products
Immunoglobulin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian lymphoblast, Hodgkins disease
Cell Line Description
Derived from a 29 year old male Caucasian with Hodgkin′s disease. One of a large series of permanent lines from leukocytes in the peripheral blood of donors with and without malignancies. Has cell-associated EBV and produces immunoglobulins. This cell line was initially thought to have been derived from Hodgkin lymphoma, but is now known to be an EBV-positive B-lymphoblastoid cell line.
Application
Tumourigenicity studies
Culture Medium
RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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