HT55 Cell Line human
85061105, human colon, Epithelial
Synonym(s):
HT55 Cells
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About This Item
UNSPSC Code:
41106514
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product name
HT55 Cell Line human, 85061105
biological source
human colon
description
Human colon carcinoma
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human colon carcinoma
Cell Line Description
Human colon epithelial cell line
Application
Tumourigenicity studies
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 9,13
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 8,9.3
TPOX: 8,9
vWA: 14,17
CSF1PO: 11
D13S317: 9,13
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 8,9.3
TPOX: 8,9
vWA: 14,17
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA and incubate until cell sheet peels off. Cells grow in islands, not monolayers; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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