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biological source
human lung
growth mode
Adherent
karyotype
Number of chromosomes 38/76
morphology
Mixed epithelial and spindle shaped
products
Epithelial membrane antigen (EMA)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure
Cell Line Description
Mero-84 was derived from a pleural effusion
Application
Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8,12
D16S539: 9
D5S818: 11
D7S820: 9,11
THO1: 9
TPOX: 9,11
vWA: 14,17
CSF1PO: 10,11
D13S317: 8,12
D16S539: 9
D5S818: 11
D7S820: 9,11
THO1: 9
TPOX: 9,11
vWA: 14,17
Culture Medium
Hams F10 + 15% FCS + 2mM Glutamine
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 48 hours
Other Notes
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Certificates of Analysis (COA)
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