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MilliporeSigma
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Key Documents

C9483

Sigma-Aldrich

Coating Stabilizer and Blocking Buffer

liquid

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

sterility

non-sterile

Quality Level

form

liquid

storage temp.

2-8°C

General description

Contains 24 ppm 5-chloro-2-methyl-4-isothiazolin-3-one and 8 ppm 2-methyl-4-isothiazolin-3-one.

Application

The solution can be used to improve the stability and function of antigens and other very unstable proteins bound to a solid phase. It can be used as supplied for very unstable proteins, or diluted 1:1 with currently used blocking agent for less labile antigens and proteins. Coating Stabilizer and Blocking Buffer has been used as a blocking agent in transamidation assay to evaluate gliadin and other modelled ligands.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

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Towards Celiac-safe foods: Decreasing the affinity of transglutaminase 2 for gliadin by addition of ascorbyl palmitate and ZnCl 2 as detoxifiers
Engstrom N, et al.
Scientific Reports, 7(1), 77-77 (2017)
N Engstrom et al.
Scientific reports, 7(1), 77-77 (2017-03-03)
Initiation of celiac disease is triggered in the gastrointestinal tract by transglutaminase 2 (TG2) assisted deamidation of gluten peptides. Deamidation is a side-reaction to transamidation and occurs if primary amines are absent. In contrast to deamidation, transamidation does not trigger

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