C8052
Cholera Toxin
from Vibrio cholerae, ≥90% (SDS-PAGE), lyophilized powder, A-B type toxin
Synonym(s):
CTX, Cholera enterotoxin, Choleragen
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
product name
Cholera Toxin from Vibrio cholerae, ≥90% (SDS-PAGE), lyophilized powder
Quality Level
Assay
≥90% (SDS-PAGE)
form
lyophilized powder
composition
Protein, ~5% Lowry-TCA
solubility
H2O: 10 mg/mL
storage temp.
2-8°C
Looking for similar products? Visit Product Comparison Guide
General description
Cholera toxin is a member of the AB5-subunit family of toxins. It consists of a single A subunit responsible for ADP-ribosylation and five B subunits arranged as a pentameric ring with cell surface receptor binding and internalization functions. The A subunit catalyzes the ADP-ribosylation of the α-subunit of the stimulatory G protein (Gαs), leading to increased adenylyl cyclase activity and cAMP levels. It also ADP-ribosylates transducing and tubulin.
Cholera toxin and its A subunit are commonly used to study signal transduction mechanisms due to their impact on adenylate cyclase. Additionally, cholera toxin acts as an adjuvant by stimulating B lymphocytes and T helper cell type 2 responses by inhibiting interleukin-12 production. The non-toxic B subunit (CTB) attaches to cells by binding to ganglioside GM1, making it a useful label for microglial cells in neurological research. It has also proved to be an excellent tracer for axonal transport studies using immunohistochemical methods.
Cholera toxin and its A subunit are commonly used to study signal transduction mechanisms due to their impact on adenylate cyclase. Additionally, cholera toxin acts as an adjuvant by stimulating B lymphocytes and T helper cell type 2 responses by inhibiting interleukin-12 production. The non-toxic B subunit (CTB) attaches to cells by binding to ganglioside GM1, making it a useful label for microglial cells in neurological research. It has also proved to be an excellent tracer for axonal transport studies using immunohistochemical methods.
Application
Cholera Toxin from Vibrio cholerae has been used as a positive control in cAMP (cyclic AMP) assay for enterotoxins. It has been added as a supplement in cell culture media of primary tumors and epithelial cells.
Cholera Toxin from Vibrio cholerae has been used in toxin neutralization assays to assess the cytotoxicity of cells.
Features and Benefits
This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
Packaging
Package size based on protein content.
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide and sodium EDTA
Reconstitution
When reconstituted at 1 mg/mL in water, solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN3, and 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 2 Oral - Acute Tox. 4 Inhalation - Aquatic Chronic 3
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Nobuhiko Kayagaki et al.
Science (New York, N.Y.), 341(6151), 1246-1249 (2013-07-28)
Gram-negative bacteria including Escherichia coli, Citrobacter rodentium, Salmonella typhimurium, and Shigella flexneri are sensed in an ill-defined manner by an intracellular inflammasome complex that activates caspase-11. We show that macrophages loaded with synthetic lipid A, E. coli lipopolysaccharide (LPS), or
The enterotoxin T (BcET) from Bacillus cereus can probably not contribute to food poisoning.
Choma C and Granum PE
FEMS Microbiology Letters, 217, 115-115 (2002)
Nicholas F Wright et al.
The Journal of comparative neurology, 521(13), 2966-2986 (2013-03-19)
Many brain structures project to both the anteroventral thalamic nucleus and the anteromedial thalamic nucleus. In the present study, pairs of different tracers were placed into these two thalamic sites in the same rats to determine the extent to which
Contribution of CXCL12 secretion to invasion of breast cancer cells.
Boimel PJ et al.
Breast Cancer Research, 14, R23-R23 (2012)
Rapid Expansion of Human Epithelial Stem Cells Suitable for Airway Tissue Engineering.
Butler CR et al.
American Journal of Respiratory and Critical Care Medicine, 194, 156-156 (2016)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service