C-12811
Human Cardiac Myocytes (HCM)
500,000 proliferating cells
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
Recommended Products
biological source
human heart (ventricles)
packaging
pkg of 500,000 cells
morphology
(myocytes)
technique(s)
cell culture | mammalian: suitable
shipped in
ambient
storage temp.
room temp
General description
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Cell Line Origin
Heart
Application
Primary Human Cardiac Myocytes (HCM) are isolated from the ventricles of the adult heart and are provided in a proliferating format. They are qualified for in vitro research on cardiac diseases and for pharmacological studies. Unlike freshly isolated rod-shaped myocytes, cultured HCM can be used for long-term experiments like investigating the long-term effects of cytokines, mechanical strain, or cell-cell interactions, as they are prepared according to a special protocol.Initially, the HCM act more like progenitor cells in that they are not yet fully differentiated. They express the markers of early stage differentiation such as GATA-4 and sarcomeric alpha-actin and have a high capacity for proliferation. When they are grown to confluency and cultivated for an extended period of time, the differentiation process begins. Markers of late differentiation (e.g. sarcomeric alpha-actinin, slow muscle myosin) are increased and the cells begin to form myotube-like structures.
Quality
Rigid quality control tests are performed for each lot of Human Cardiac Myocytes. They are tested for cell morphology, adherence rate, and cell viability. Furthermore, flow cytometric analyses for cell-type specific markers, e.g. sarcomeric a-actinin and slow muscle myosin, are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Warning
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
Subculture Routine
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Other Notes
Recommended Plating Density: 10000 - 15000 cells per cm2Passage After Thawing: P2Tested Markers: Sarcomeric alpha-actinin positive; Slow muscle myosin positiveGuaranteed population doublings: >15
Recommended products
Recommended Primary Cell Culture Media:Link
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Articles
Primary human cardiac myocytes isolated from normal human ventricle tissue of the adult heart, plus optimized myocyte media. Protocols for handling, cell passaging, media, and product use.
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