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B9398

Sigma-Aldrich

Anti-Bovine Serum antibody produced in rabbit

whole antiserum

Synonym(s):

Anti-Serum, bovine

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About This Item

MDL number:
MFCD00162174
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

bovine

technique(s)

direct immunofluorescence: 1:160 using human peripheral blood lymphocytes
immunoelectrophoresis: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

bovine ... Alb(280717)

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General description

Bovine serum consists of fats, plasma proteins, growth factors, peptides, carbohydrates, hormones and inorganic substances.

Specificity

Rabbit Anti-Bovine Whole Serum is determined to have strong reactivity with bovine serum proteins. No reactivity with horse or pig serum proteins is observed.

Application

Anti-Bovine Serum antibody produced in rabbit may be used to prepare anti-BSA-BSA complexes that enhance the relative procoagulant activity of leukocytes. It has been used in
  • immunoelectrophoresis
  • immunodiffusion
  • enzyme linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Bovine serum is required for the in vitro growth of cells. Bovine serum albumin (BSA) is one of the important constituents of bovine serum.

Other Notes

Antiserum adsorbed with equine and porcine serum proteins

Physical form

Supplied as a liquid containing 0.1% sodium azide (see MSDS) as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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H Rothberger et al.
The Journal of clinical investigation, 59(3), 549-557 (1977-03-01)
In a variety of immunologic diseases, fibrin-fibrinogen and immune complexes deposit in areas of tissue damage. However, the mechanisms which initiate fibrin-fibrinogen deposition have not been clarified. We find that the procoagulant activity of human leukocytes is markedly increased after
Yin Zhang et al.
BioMed research international, 2016, 5013409-5013409 (2016-11-11)
Previously we have shown that (-)-epigallocatechin gallate (EGCG) can induce nonapoptotic cell death in human hepatoma HepG2 cells only under serum-free condition. However, the underlying mechanism for serum in determining the cell fate remains to be answered. The effects of

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