B4287
Bovine Serum Albumin
heat shock fraction, protease free, suitable for hybridization, pH 7, ≥98%
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
Sign Into View Organizational & Contract Pricing
All Photos(4)
About This Item
Recommended Products
biological source
bovine
Quality Level
grade
for molecular biology
product line
BioReagent
Assay
≥98%
form
lyophilized powder
mol wt
~66 kDa
purified by
heat shock fractionation
packaging
poly bottle of
origin
USA origin
technique(s)
ELISA: suitable
electrophoresis: suitable
western blot: suitable
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
Protease free
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
Looking for similar products? Visit Product Comparison Guide
Application
A non-acetylated protein suitable as a blocking agent in Southern blotting. If your application requires nuclease-free BSA, we recommend our acetylated BSA for these critical applications.
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Features and Benefits
- Protease free
- Suitable for blocking non-specific protein binding sites in hybridizations
- Heat shock fractionated
Preparation Note
Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Line Verckist et al.
Respiratory research, 19(1), 207-207 (2018-10-28)
The microenvironment (ME) of neuroepithelial bodies (NEBs) harbors densely innervated groups of pulmonary neuroendocrine cells that are covered by Clara-like cells (CLCs) and is believed to be important during development and for adult airway epithelial repair after severe injury. Yet
Matthew L Fisher et al.
Molecular carcinogenesis, 55(12), 2024-2036 (2015-12-24)
Melanoma is a metastatic cancer associated with poor survival. Here, we study a subpopulation of melanoma cancer cells displaying melanoma cancer stem cell (MCS cells) properties including elevated expression of stem cell markers, increased ability to survive as spheroids, and
Gautam Adhikary et al.
Carcinogenesis, 36(7), 800-810 (2015-05-15)
Polycomb group proteins, including Ezh2, are important candidate stem cell maintenance proteins in epidermal squamous cell carcinoma. We previously showed that epidermal cancer stem cells (ECS cells) represent a minority of cells in tumors, are highly enriched in Ezh2 and
Balázs Rada et al.
Journal of immunology (Baltimore, Md. : 1950), 181(7), 4883-4893 (2008-09-20)
The dual oxidase-thiocyanate-lactoperoxidase (Duox/SCN(-)/LPO) system generates the microbicidal oxidant hypothiocyanite in the airway surface liquid by using LPO, thiocyanate, and Duox-derived hydrogen peroxide released from the apical surface of the airway epithelium. This system is effective against several microorganisms that
Ignacio Izeddin et al.
PloS one, 6(1), e15611-e15611 (2011-01-26)
The actin cytoskeleton of dendritic spines plays a key role in morphological aspects of synaptic plasticity. The detailed analysis of the spine structure and dynamics in live neurons, however, has been hampered by the diffraction-limited resolution of conventional fluorescence microscopy.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service