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A0156

Sigma-Aldrich

N-(4-Aminobutyl)-N-ethylisoluminol

≥90%, powder

Synonym(s):

4-(N1-Ethyl-4-aminobutylamino)phthalic hydrazide, 6-[N-(4-Aminobutyl)-N-ethylamino]-2,3-dihydro-1,4-phthalazinedione, ABEI

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About This Item

Empirical Formula (Hill Notation):
C14H20N4O2
CAS Number:
66612-29-1
Molecular Weight:
276.33
Beilstein:
920895
MDL number:
MFCD00010559
UNSPSC Code:
12352204
PubChem Substance ID:
24890404
NACRES:
NA.83

product name

N-(4-Aminobutyl)-N-ethylisoluminol, ≥90%

Assay

≥90%

form

powder

mp

259-260 °C (lit.)

solubility

glacial acetic acid: 50 mg/mL, clear, colorless to yellow

storage temp.

2-8°C

SMILES string

CCN(CCCCN)c1ccc2C(=O)NNC(=O)c2c1

InChI

1S/C14H20N4O2/c1-2-18(8-4-3-7-15)10-5-6-11-12(9-10)14(20)17-16-13(11)19/h5-6,9H,2-4,7-8,15H2,1H3,(H,16,19)(H,17,20)

InChI key

LEOJISUPFSWNMA-UHFFFAOYSA-N

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Features and Benefits

Efficient chemiluminescent NH2-coupling reagent for detection of a wide variety of proteins down to the picomole range.

Other Notes

Reportedly, the use of this material in chemiluminescent assays has distinct advantages over conventional radioimmunoassay.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shulin Zhao et al.
Chemical communications (Cambridge, England), 48(5), 699-701 (2011-12-03)
We report on a novel strategy to improve microfluidic immunoassay sensitivity by introducing chemiluminescence resonance energy transfer (CRET) into the immunoreactions. The proposed CRET-based immunoassay for estradiol (E2, as a model analyte) is one of the most sensitive immunoassay with
L Farina et al.
Journal of bioluminescence and chemiluminescence, 4(1), 587-593 (1989-07-01)
We have developed a two-site immunoassay for human prolactin using two monoclonal antibodies: one of them immobilized on the solid phase and the other labelled with biotin. The serum is incubated simultaneously with the antibody-coated bead, the biotinylated antibody and
O M Steijger et al.
Journal of chromatography, 615(1), 97-110 (1993-05-19)
N-(4-Aminobutyl)-N-ethylisoluminol was used for labelling of carboxylic acids. The derivatization reaction was carried out with 1-hydroxybenzotriazole as pre-activator of the carboxylic acid function and N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide as the coupling reagent. Optimum conditions for the derivatization were determined by using factorial design
R M Lequin et al.
Journal of immunoassay, 7(3), 169-179 (1986-01-01)
Chemiluminescent labels have been shown to be interesting alternatives to radioisotope labels. Disadvantages of the latter are preparation of e.g. labelled protein/peptides every four to six weeks, and problems with storage and disposal. Amino-Butyl-Ethyl-Isoluminol(ABEI) was attached to the alpha-amino function
Shulin Zhao et al.
Analytical biochemistry, 393(1), 105-110 (2009-06-23)
A microchip electrophoresis (MCE) method with chemiluminescence (CL) detection was developed for the determination of carnosine-related peptides, including carnosine, homocarnosine, and anserine, in biological samples. A simple integrated MCE-CL system was built to perform the assays. The highly sensitive CL
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