83330

Pyruvate Kinase from rabbit muscle

crystalline suspension in 3.8 M ammonium sulfate solution, ~350 U/mg protein (10-40 mg/ml)

• Synonym(s): ATP:pyruvate 2-O-phosphotransferase, PK
• CAS Number: 9001-59-6
• Enzyme Commission number: 2.7.1.40 (BRENDA, IUBMB)
• EC Number: 232-616-2
• MDL number: MFCD00081946
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

Properties

• form: suspension
• quality: crystalline suspension in 3.8 M ammonium sulfate solution
• specific activity: ~350 U/mg protein (10-40 mg/ml)
• mol wt: M_r ~230000
• pH: ~6
• foreign activity: lactate dehydrogenase ≤0.01%
• storage temp.: 2-8°C

Description

Biochem/physiol Actions

Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA₂₈₀ = 0.54 for 1 mg(p)/ml, 1 cm path
Reported K_M values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.

Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate to pyruvate, whitch is the rate-limiting final step in glycolysis.

Unit Definition

1 U corresponds to the amount of enzyme which catalyzes the transphosphorylation of 1 μmol PEP per min at pH 7.4 and 25 °C

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves