45245
Eosin 5-isothiocyanate
≥95.0% (UV)
Synonym(s):
EITC
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About This Item
Empirical Formula (Hill Notation):
C21H7Br4NO5S
CAS Number:
Molecular Weight:
704.96
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
Recommended Products
Assay
≥95.0% (UV)
fluorescence
λex 521; λem 544 (pH 9.0)
storage temp.
2-8°C
SMILES string
OC(=O)c1cc(ccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c(O)c(Br)cc24)N=C=S
InChI
1S/C21H7Br4NO5S/c22-12-4-10-14(8-2-1-7(26-6-32)3-9(8)21(29)30)11-5-13(23)18(28)16(25)20(11)31-19(10)15(24)17(12)27/h1-5,27H,(H,29,30)
InChI key
MCXYFYWNOWPDMA-UHFFFAOYSA-N
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Application
Eosin 5-isothiocyanate (EITC) is a fluorescence probe. EITC tagged molecules may be used together with fluorescein-5′-isothiocyanate (FITC) tagged molecules in fluoresce resonance energy transfer (FRET) based assays.
Other Notes
Acceptor fluorophore. Measurement of rotational diffusion of proteins; In fluorescence energy transfer studies
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Ya-lan Wu et al.
Guang pu xue yu guang pu fen xi = Guang pu, 24(12), 1597-1600 (2005-04-15)
In this paper, the medium (pH, organic solvent and surfactant) effects on fluorescence of eosin-5-isothiocyanate (eosin-ITC) have been studied in detail. The results show that the pH and polarity of system studied are two great important influence factors on fluorescence
M Schindler et al.
The Journal of cell biology, 92(3), 742-746 (1982-03-01)
Chemical modification of amino groups in matrix porin solubilized and purified from outer membranes of Escherichia coli in beta-octylglucoside was performed with eosin isothiocyanate and citraconic anhydride. At pH 7 8.5, the former reagent labeled a single amino group in
R Wagner et al.
European journal of biochemistry, 173(3), 561-568 (1988-05-02)
Phosphoenolpyruvate carboxylase, purified from maize leaves, is rapidly inactivated by the fluorescence probe dansyl chloride. The loss of activity can be ascribed to the covalent modification of an R-NH2 group, presumably the epsilon-NH2 group of lysine. Analysis of the data
R Mittal et al.
Biochemistry, 33(33), 10178-10184 (1994-08-23)
We have used resonance energy transfer to read out the interactions of the alpha subunit of transducin (alpha T) with the transducin beta gamma subunit complex (beta gamma T) and to compare the rate of aluminum fluoride-induced alpha T activation
Yan Fu et al.
Journal of immunological methods, 289(1-2), 57-64 (2004-07-15)
A solid substrate room temperature phosphorescence immunoassay (SS-RTP-IA) for the determination of human complement3 (C3) based on a sandwich type assay and a labelled avidin-biotin (LAB) type assay was described. The anti-human complement3 and avidin were labeled with eosin5-isothiocyanate. On
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