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W2261

Millipore

WL nutrient broth

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Wallerstein Laboratory Nutrient Broth

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

5.5±0.2 (25 °C)

application(s)

bioburden testing
food and beverages
microbiology

suitability

selective for Candida spp.
selective for Escherichia coli
selective for Lactobacillus spp.
selective for Pichia spp.
selective for Proteus spp.
selective for Saccharomyces spp.
selective for Zygosaccharomyces spp.
selective for coliforms

Application

Recommended for the cultivation of bacteria encountered in breweries and industrial fermentations.

Components

Ingredients (g/L)
Casein enzymatic hydrolysate, 5.00
Yeast extract, 4.00
Dextrose, 50.00
Monopotassium phosphate, 0.55
Potassium chloride, 0.425
Calcium chloride, 0.125
Manganese sulfate, 0.125
Ferric chloride, 0.0025
Manganese sulfate, 0.0025
Bromocresol green, 0.022

Preparation Note

Suspend 60.25 g of WL Nutrient Broth in 1000 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. If desired to obtain a pH of 6.5 add a 1% solution of sodium bicarbonate (Cat. No. 71627).

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCBX4269
BCBW1158
BCBV4178
BCBR7665V
BCBN3867V

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Articles

Microbiological Media and Detection

Molecular biology-based methods, like PCR, can be used for lactobacilli detection.

Culture Method for Beer Quality Control

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.

Probiotics and Human Health

Today, diverse studies report the benefits of probiotics, such as inhibitory effects on pathogens, aid in the management or prevention of chronic intestinal inflammatory diseases or atopic syndromes, and support to the immune system. Potential beneficial applications abound, researchers continue to evaluate the effictiveness and clarify the mechanisms of action of probiotics.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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