UPH2O

Ultra pure DNA-free Water

DNA-free, Nuclease-free water

• UNSPSC Code: 41106229

Properties

• Quality Level: 300
• form: liquid
• feature: DNA free; RNase and DNase free (also Nickase free)
• packaging: pkg of 1.5 mL
• storage condition: room temperature
• technique(s): DNA sequencing: suitable; RT-PCR: suitable; cDNA synthesis: suitable; qPCR: suitable
• dilution: (Molecular assays)
• shipped in: ambient

Description

General description

Ultra pure DNA-free Water is generated using a Milli-Q^® IQ 7000 Ultrapure Water Purification System, filter-sterilized and UV-treated, and undergoes stringent quality control testing to ensure that it is free of detectable levels of DNA, DNase, RNase, and Nickase to reduce the risk of false positive and false negative results in molecular diagnostic assays. Ultra pure DNA-free Water should be used with other ultra pure DNA-free reagents, such as Ultra pure JumpStart^™ Taq DNA Polymerase, Ultra pure High-temp Reverse Transcriptase, and accompanying Ultra pure RT-PCR buffers, to minimize false positive and negative results.
Ultra pure DNA-free Water was designed for use in highly-regulated industries in applications that require a high level of performance, lot-to-lot consistency, and high sensitivity without false results from reagent-borne contamination while meeting industry-driven critical regulations. To meet this requirements we have increased the quality level of our products. The M-Clarity Program defines product quality. It provides transparency so that you can choose, with confidence, suitable products that meet your needs with respect to quality and regulatory compliance, transparency, change notification, and documentation.

Application

Ultra pure DNA-free Water can be used for:

• Use in molecular assays
• DNA sequencing
• cDNA synthesis
• PCR and qPCR
• For further manufacture

Features and Benefits

• DNA-free
• DNase-free, RNase-free, and nickase-free
• Filter-sterilized
• UV-treated, not DEPC-treated
• Ideal for high throughput molecular applications
• Enhanced change control and quality agreement

Packaging

Ultra pure DNA-free Water is provided in larger SKU sizes for ease of use in high throughput RT-PCR applications and for further manufacture. Ultra pure DNA-free Water should be used with other ultra pure DNA-free reagents, such as Ultra pure JumpStart^™ Taq DNA Polymerase, Ultra pure High-temp Reverse Transcriptase, and accompanying Ultra pure RT-PCR buffers, to minimize false positive and negative results.

Other Notes

Quality testing ensures the absence of detectable levels of contaminating DNA (<=1 copy of prokaryotic gDNA, <=0.1 copy of eukaryotic gDNA, and <=10 copies of plasmid DNA per 100 units of enzyme) as detected by qPCR using universal primers against 16S capable of detecting > 500,000 prokayotes, 18S capable of detecting > 110,000 eukaryotes, and Ori capable of detecting > 25,000 plasmids, respectively.

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

Milli-Q is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg