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M8302

Millipore

MacConkey Agar with Crystal violet, Sodium chloride and 0.15% Bile salts

NutriSelect® Plus, suitable for microbiology

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

pH

7.2±0.2 (25 °C)

application(s)

environmental
food and beverages
microbiology

suitability

selective and differential for Enterobacter spp.
selective and differential for Enterococcus spp.
selective and differential for Escherichia coli
selective and differential for Klebsiella spp.
selective and differential for Proteus spp.
selective and differential for Salmonella spp.
selective and differential for Shigella spp.
selective and differential for Streptococcus spp.
selective and differential for coliforms
selective and differential for enterobacteriaceae

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Application

A differential media recommended for the selection and recovery of Enterobacteriaceae.

Components

Components (g/L)
Peptic digest of animal tissue 20.0
Lactose 10.00
Bile salts 1.50
Sodium chloride 5.00
Crystal violet 0.001
Neutral red 0.05
Agar 15.00

Preparation Note

Suspend 51.55 g of MacConkey Agar with Crystal Violet, Sodium Chloride and 0.15% Bile Salts in 1000 ml of distilled water. Heat to boiling, with gentle swirling to dissolve the agar completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Avoid overheating. Cool to 45-50°C and pour into sterile petri plates.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG3230
BCCG3247
BCCF6219
BCCC6759
BCCB7690

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United States Pharmacopeia/National Formulary (1985)
Caroline S Achard et al.
Microorganisms, 8(10) (2020-10-18)
Antibiotic resistance of microbes thriving in the animal gut is a growing concern for public health as it may serve as a hidden reservoir for antibiotic resistance genes (ARGs). We compared 16 control piglets to 24 piglets fed for 3
Ilenys M Pérez-Díaz et al.
Food microbiology, 77, 10-20 (2018-10-10)
Limited documentation of the cucumber fermentation microbiome has impeded the understanding of the role of microbes on the quality of finished products. We characterized the microbiome of fresh and fermented cucumber samples using culture dependent and independent techniques, with an

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Differentiation and Identification Media for Salmonella

Salmonella contamination is the second leading cause of food-borne illness worldwide. Controlling outbreaks of Salmonella is an important task for food regulators, restaurants and the food industry in general. The Salmonella family includes over 2,300 serotypes of bacteria, but two types, Salmonella enteritidis and Salmonella typhimurium, are responsible for about half of all human infections. Most outbreaks of Salmonella are traced back to dairy, poultry and meat products, but Salmonella can grow on nearly any food. Chicken, eggs and their derivative products are particularly high risk.

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