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75605

Millipore

m-CP Agar Base

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Membrane Clostridium perfringens Agar Base

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
bromocresol purple, 0.04 g/L
L-cysteine hydrochloride, 1 g/L
ferric chloride 6H2O, 0.09 g/L
indoxyl-β-D-glucoside, 0.06 g/L
magnesium sulfate 7H2O, 0.1 g/L
sucrose, 5 g/L
tryptose, 30 g/L
yeast extract, 20 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.6±0.2 (25 °C)

application(s)

environmental
food and beverages
water monitoring
microbiology

storage temp.

2-8°C

suitability

selective and differential for Clostridium spp.

Application

The m-CP Agar Base with selective supplement is recommended by the Directive of the Council of the European Union 98/83/EC for isolation and enumeratin of Clostridium perfringens from water samples using membrane filtration technique.

Preparation Note

Suspend 35.6 g in 485 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 1 bar pressure (121 °C) for 15 minutes. Cool to 50 °C. Aseptically add the rehydrated contents of 1 vial of M-CP Selective Supplement I (Cat. No. 51962) and 1 vial of M-CP Selective Supplement II (Cat. No. 82265). Mix well and pour into sterile petri plates.

Other Notes

Medium for the assay of water for Clostridium perfringens; EU 98/83/EC Council 3rd November 1998 on quality of water intended for human consumption

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCF2831
BCCB1574
BCBX1057
BCBT7452
BCBD4182V

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Jens Tschmelak et al.
Biosensors & bioelectronics, 20(8), 1509-1519 (2005-01-01)
A novel analytical system AWACSS (Automated Water Analyser Computer Supported System) based on immunochemical technology has been evaluated that can measure several organic pollutants at low nanogram per litre level in a single few-minutes analysis without any prior sample pre-concentration
R Armon et al.
Canadian journal of microbiology, 34(1), 78-79 (1988-01-01)
Medium m-CP, designed for the isolation of Clostridium perfringens from water samples, contains indoxyl beta-D-glucoside, an expensive chemical that is present at a high concentration in this medium. The use of m-CP with three concentrations of indoxyl beta-D-glucoside was tested

Articles

Clostridia Diagnostic

Clostridia are relatively large, gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism.

Clostridium perfringens, their Properties and their Detection

An article regarding the Role of Clostridium perfringens and their detection, identification, and differentiation from Sigma-Aldrich.com

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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