67432

Campylobacter Millichrome^™ plus Supplement

pkg of 5 L, suitable for microbiology

• Synonym(s): Campylobacter chromogenic Supplement
• UNSPSC Code: 41171614
• NACRES: NA.74

Properties

• sterility: sterile; 0.45 μm filtered
• Quality Level: 200
• form: dry powder
• shelf life: limited shelf life, expiry date on the label
• packaging: pkg of 5 L
• technique(s): microbiological culture: suitable
• solubility: sterile water: 21 mg/mL
• application(s): food and beverages; microbiology
• shipped in: ambient
• storage temp.: 2-8°C
• suitability: selective for bacteria: Campylobacter spp.

Description

General description

Campylobacter Millichrome^™ plus supplement is a selective media supplement for the detection of thermotolerant Campylobacter. The supplement provides additional selectivity to the Campylobacter Millichrome^™ plus Agar Base. Millichrome^™ chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. The package contains 1 vial of supplement sufficient for 5 liters of final media. The supplement must be added to the melted Campylobacter Millichrome^™ plus Agar Base at 45-50 °C.

Application

Campylobacter Millichrome^™ plus supplement is a selective chromogenic culture media supplement intended for use in the qualitative direct detection, differentiation and presumptive identification of pathogenic biotypes of thermotolerant Campylobacter from various samples

Components

Composition in g/l:
(1 vial is sufficient for 5 L of final media)
Chromogenic and selective mix 1.05 g

Preparation Note

• In a transparent vessel, add 210 mg of Campylobacter Millichrome^™ plus Supplement to 10 ml purified water.
• Swirl well until complete dissolution.
• Filter to sterilize at 0.45 μm.
• Add the 10 ml of the supplement solution to 1 liter of the melted Campylobacter Chromocult^® plus Agar base at 45-50 °C.
• Swirl or stir gently to homogenize.

Analysis Note

The Campylobacter colonies are colored red after 48 h of incubation at an optimal growth temperature of 42°C, whereas the growth of other species is inhibited or forms blue colonies after incubation at the same temperature.

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS02,GHS08,GHS05
• Signal Word: Danger
• Hazard Statements: H228,H317,H318,H334
• Precautionary Statements: P210 - P240 - P241 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1 - Flam. Sol. 1 - Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 4.1B - Flammable solid hazardous materials
• WGK: WGK 3