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G6PDHII-RO

Roche

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

grade II, from yeast

Synonym(s):

G6P-DH, Glucose-6-Phosephate Dehydrogenase

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About This Item

Enzyme Commission number:
1.1.1.49 (BRENDA, IUBMB)
UNSPSC Code:
12352204

biological source

yeast

Quality Level

100

form

suspension

specific activity

~140 units/mg protein (At 25 °C with glucose-6-P as the substrate; standardized with BSA.)

packaging

pkg of 2 mL (10127671001 [5 mg/mL])
pkg of 5 mL (10737232001 [5 mg/mL])

manufacturer/tradename

Roche

optimum pH

9.2

pH range

5-11

shipped in

wet ice

General description

Glucose-6-Phosphate Dehydrogenase (G6PD) is a ubiquitous enzyme which acts as a catalyst in producing pentose. It also produces NADPH for various biosynthetic and detoxification reactions. Sometimes G6PD provides an alternative to main glycolytic pathway for glucose utilization. It also has its application as DNA markers on X-chromosome.

Application

Glucose-6-Phosphate Dehydrogenase (G6P-DH) is a component of cofactor recycling systems for NADPH. Additional reagent glucose-6-phosphate.
It was used in enzymatic determination of C6 phosphorylation of glycogen.

Quality

Contaminants: <0.001% CK, <0.05% GR, <0.01% 6-PGDH and PGluM each, <0.02% HK, <0.002% PGI

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6.0

Preparation Note

Activator: Mg2+ (5 to 10 mM)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

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Anna A DePaoli-Roach et al.
The Journal of biological chemistry, 290(2), 841-850 (2014-11-25)
Glycogen is a branched polymer of glucose that acts as an energy reserve in many cell types. Glycogen contains trace amounts of covalent phosphate, in the range of 1 phosphate per 500-2000 glucose residues depending on the source. The function

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